Serum Protein Expression Profiling for Cancer Detection: Validation of a SELDI-Based Approach for Prostate Cancer Article

Open Access Industry Collaboration

author list

  • Grizzle, WE; Adam, BL; Bigbee, WL; Conrads, TP; Carroll, C; Feng, ZD; Izbicka, E; Jendoubi, M; Johnsey, D; Kagan, J; Leach, RJ; McCarthy, DB; Semmes, OJ; Srivastava, S; Srivastava, S; Thompson, IM; Thornquist, MD; Verma, M; Zhang, Z; Zou, ZQ


  • Multiple studies have reported that analysis of serum and other bodily fluids using surface enhanced laser desorption/ionization time of flight mass spectroscopy (SELDI-TOF-MS) can identify a “fingerprint” or “signature” of spectral peaks that can separate patients with a specific disease from normal control patients. Ultimately, classification by SELDI-TOF-MS relies on spectral differences in position and amplitude of resolved peaks. Since the reproducibility of quantitation, resolution and mass accuracy of the SELDI-TOF-MS, or any high throughput mass spectrometric technique, has never been determined this method has come under some skepticism as to its clinical usefulness. This manuscript describes a detailed design of a three-phase study to validate the clinical usefulness of SELDI-TOF-MS in the identification of patients with prostatic adenocarcinoma (PCA). At the end of this validation study, the usefulness of the general SELDI-TOF-MS approach to identifying patients with PCA will be demonstrated and how it compares with PCA diagnosis by measuring prostate specific antigen.


  • Grizzle, William E.
  • Adam, Bao-Ling
  • Bigbee, William L.
  • Conrads, Thomas P.
  • Carroll, Christopher
  • Feng, Ziding
  • Izbicka, Elzbieta
  • Jendoubi, Moncef
  • Johnsey, Donald
  • Kagan, Jacob
  • Leach, Robin J.
  • McCarthy, Diane B.
  • Semmes, IV, O. John
  • Srivastava, Shiv
  • Srivastava, Sudhir
  • Thompson, Ian M.
  • Thornquist, Mark D.
  • Verma, Mukesh
  • Zhang, Zhen
  • Zou, Zhiqiang

publication date

  • 2004

published in



  • prostate adenocarcinoma
  • validation

number of pages

  • 10

start page

  • 185

end page

  • 195


  • 19


  • 4-5